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Test Code Syphilis Ab Syphilis Antibody, IgG and IgM

Patient Preparation

No special patient preparation required

Collection Method

Venous collection

Minimum Volume

  • Adults: 2 ml
  • Pediatric: 1 mL

Container

Gold Top / Serum Separator / Additive Free

Preferred Specimen

Gold Top SST Serum

Acceptable Specimens

Serum


  • Gold Top SST

  • Red Top

Plasma

  • Light Green Top (Lithium Heparin)

Reference or Target Ranges

Nonreactive

Reportable Units

Reactive/Nonreactive

Critical Value

Not defined for this assay

Reasons for Rejection



<!-- Rejection-->
Hemolysis Threshold
 500 mg/dL
Icterus Threshold
 40 mg/dL
Lipemia Threshold
 2000 mg/dL
Other
 QNS, contaminated, improperly Labeled, improper specimen type

Specimen Stability



<!-- Stability -->
Ambient
 5 days
Refrigerated (4°C to 8°C)
 14 days
Frozen(-40°C to 0°C)
 12 months

Performance Information



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Days and Time Performed
 24/7
Expected Turn Around Time
 Stat: 1 hour
Routine: 4 hours
Stat Availability
 Yes
Performing Bench
 WCH Chemistry
Methodology/Method Description
 Roche Cobas – Two site sandwich assay

Reflex Testing

All positive results will reflex to RPR rapid testing for titer. If syphilis results are reactive and RPR results are negative, TPPA-Mayo will be sent out for testing. This test cannot be used for congenital syphilis, except indirectly if the mother of a newborn is positive with this and the reflexed RPR on the same sample.

CPT Codes

86780