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Test Code SPEP Serum Protein Electrophoresis w/ Reflex to Immunotyping 

Clinical Use

Accurate relative quantification of individual protein fractions and  Qualitative detection and the characterization of monoclonal proteins. 

Specimen

Venously collect a Gold top, serum seperator tube (preferred).

 

A Red Top serum tube may also be used. 

Patient Preparation

No special patient preparation required. 

Reference Range

Protein Zone 

Reference % Values 

Reference Protein Values (g/dL) 

Albumin 

55.8 – 66.1 

3.2 – 5.4

Alpha-1 globulins 

2.9 – 4.9 

0.2 – 0.4 

Alpha-2 globulins 

7.1 – 11.8 

0.4 – 1.0

Beta-1 globulins 

4.7 - 7.2 

0.3 – 0.6

Beta-2 globulins 

3.2 – 6.5 

0.2 – 0.5

Gamma globulins 

11.1 - 18.8 

0.6 – 1.5

 

Immunotyping: No monoclonal immunoglobin present 

Reportable Units

Fraction percent (%) and g/dL

Critical Values

None for this assay

Specimen Stability

Ambient: 3 days
Refrigerated (2 - 8)°C:  10 days
Frozen: (0°C and below)  2 months

 

Rejection Criteria

Hemolysis:  Grossly visible
Icterus:  N/A
Lipemia:  N/A 
Other:  QNS, Improper sample and/or handling. Other reasons deemed appropriate by laboratory staff. 

 

Performance Information

Days and Times:  M - F (8:00-1630)
Expected Turnaround Time:  48hrs (excluding weekends) 
Stat Availability:  None 
Performing Lab:  AP Green Special Chemistry 
Instrument/Methodology:  Capillary Electrophoresis by Sebia Capillarys 3

 

CPT Codes

84165, 84155; 86320(if reflexed)