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Test Code MTX Methotrexate Level

Patient Preparation

No special patient preparation required

Special Collection Instructions

Customarily, draw at 24 hour intervals after start of infusion; although earlier and more frequent monitoring may be indicated.

Collection Method

Venous collection

Minimum Volume

1 mL

Container

Lt Green / Plasma Separator / Lithium Heparin

Preferred Specimen

Light Green Top (lithium heparin) plasma

Acceptable Specimens

Serum:

  • Gold Top SST
  •  
  • Red Top


Plasma:

 

  • Light Geen Top (lithium heparin)
  • Purple or Pink Top(K2/K3 EDTA)
  • Dark Green Top (sodium heparin)

 

Reference or Target Ranges

<0.05 umol/L

Therapeutic ranges depend on specimen collection time after dose and protocol applied. 

Reportable Units

umol/L

Critical Value

N/A

Reasons for Rejection

 


<!-- Rejection-->

 

Hemolysis Threshold 1000 mg/dL
Icterus Threshold 60 mg/dL
Lipemia Threshold 700 mg/dL
Other QNS, contaminated, improperly Labeled, improper specimen type

Specimen Stability

 


<!-- Stability -->

 

Ambient 8 hours
Refrigerated (4°C to 8°C) 7 days
Frozen(-40°C to 0°C) 12 months

Performance Information

 


<!-- Performance information -->

 

Days and Time Performed 24/7
Expected Turn Around Time Stat: 1 hour
Routine: 4 hours
Stat Availability Yes
Performing Bench UH Chemistry
Methodology/Method Description Siemens Atellica - ImmunoAssay

CPT Codes

80204