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Test Code CD4 Flow CD4/CD8 Subsets for Immune Monitoring, Blood

Useful For

Please see the Flow Cytometry Flow Chart for ordering instructions
Serial monitoring of CD4 T cell count in HIV-positive patients
Useful for follow-up and diagnostic evaluation of primary cellular immunodeficiency’s, including severe combined immunodeficiency
T-cell immune monitoring following immunosuppressive therapy for transplantation, autoimmunity, and other immunological conditions where such treatment is utilized

Patient Preparation

No special patient preparation required

Collection Method

Venous draw

Minimum Volume

2 mL

Container

Purple (EDTA)

Preferred Specimen

Whole Blood

Acceptable Specimens

CD4/CD8 counts may also be performed on BAL specimens.

Reference or Target Ranges

Age-appropriate reference values provided on report

Reportable Units

cells/μL

Critical Value

Not defined for this assay

Reasons for Rejection

 

Hemolysis Threshold N/A
Icterus Threshold N/A
Lipemia Threshold N/A
Other QNS, contaminated, improperly labled, improper specimen type, specimen clotted

Specimen Stability

Ambient 24 hours
Refrigerated (4°C to 8°C) Do not refrigerate
Frozen(-40°C to 0°C) Do not freeze

Performance Information

 


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Days and Time Performed Monday – Friday 9AM – 5PM
 
Expected Turn Around Time 24 hours
Stat Availabilty No
Performing Bench Flow Cytometry
Methodology/Method Description BD FACSCanto II – Flow Cytometry, Dual Platform (CBC w. BD multitest CD3/CD8/CD45/CD4)

Additional Information/Important Notes

For CD4/8 counts on BAL specimens, select “blood” under specimen type. Under order comments free form type “BAL”

CPT Codes

86359;86360