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Test Code AFP Alpha Fetoprotein

Patient Preparation

No special patient preparation required

Collection Method

Venous collection

Minimum Volume

  • Adults: 1 mL
  • Pediatric: 0.6 mL

Container

Gold Top / Serum Separator / Additive Free

Preferred Specimen

Gold Top SST

Acceptable Specimens

Serum:

  • Gold Top SST
  • Red Top

Plasma:

  • Light Geen Top (lithium heparin)
  • Pink Top (K3 EDTA)
  • Dark Green Top (sodium heparin)

 

Reference or Target Ranges

≤8.30 ng/mL

Reportable Units

ng/mL

Critical Value

Not defined for this assay

Reasons for Rejection

 

Hemolysis Threshold 2200 mg/dL
Icterus Threshold 65 mg/dL
Lipemia Threshold 1500 mg/dL
Other QNS, contaminated, improperly Labeled, improper specimen type

Specimen Stability

 

Ambient 1 day
Refrigerated (4°C to 8°C) 7 days
Frozen(-40°C to 0°C) 3 months

Performance Information

 

Days and Time Performed 24/7
Expected Turn Around Time Stat: 1 hour
Routine: 4 hours
Stat Availability Yes
Performing Bench WCH Chemistry
Methodology/Method Description Roche Cobas – 2 Step incubation sandwich assay

Additional Information/Important Notes

The measured AFP value of a patient’s sample can vary depending on the testing procedure used. The laboratory finding must therefore always contain a statement on the AFP assay method used. AFP values determined on patient samples by different testing procedures cannot be directly compared with one another and could be the cause of erroneous medical interpretations. The University system uses the Roche Cobas 6000 for all AFP testing.

CPT Codes

82105